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Investigating Human Health Using the ELISA

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In this experiment, students will perform an Enzyme-Linked Immunosorbent Assay (ELISA) to examine the impact of this powerful test on human health. Antibodies will be used to detect minuscule amounts of antigens and determine the status of simulated samples. Three different scenarios can be explored, including pregnancy testing, early detection of heart attacks, and identification of gluten in food products.

• Explore the power and versatility of the Enzyme-Linked Immunosorbent Assay (ELISA)
• Understand antigen-antibody interactions
• Perform simulated ELISAs with controls and unknown samples
• Choose from three human health scenarios or, as a class, do all three!


Group Size:

For 10 lab groups

Time Required:

Complete in 50 minutes

Kit Includes:

Instructions, 10X ELISA wash buffer, ELISA dilution buffer, antigen (lyophilized), primary antibody (lyophilized), secondary antibody (lyophilized), ABTS substrate (lyophilized), & ABTS reaction buffer, small transfer pipets, microtiter plate, 15 mL conical tubes, & snap-top microcentrifuge tubes

All You Need:

Automatic micropipettes with tips (recommended), paper towels, distilled or deionized water, beakers or flasks, disposable lab gloves, & safety goggles



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