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Restriction Enzyme Cleavage of Plasmid and Lambda DNA

$890.00

ABOUT THIS PRODUCT:

Plasmid and lambda DNA are pre-digested with restriction enzymes – endonucleases that recognize and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

• Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences
• Understand how electrophoresis separates differently sized molecules
• Perform agarose gel electrophoresis
• Load, run, analyze, and compare six restriction enzyme cleavage products (uncut, EcoRI digested, BglI digested lambda DNA and plasmid DNA)

描述

Group Size:

For 8 gels


Time Required:

Complete in 45 minutes


Kit Includes:

Instructions, Ready-to-Load QuickStrip™ DNA Samples, UltraSpec-Agarose™, Electrophoresis Buffer (50X), FlashBlue™ DNA Stain, InstaStain® Blue Cards, Practice Gel Loading Solution, & Disposable Pipets.


All You Need:

DNA ElectrophoresisMicropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.


Storage:

Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

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