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Expanding Our Testing: Using ELISA to Detect COVID-19

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Due to the worldwide spread of the respiratory disease COVID-19, scientists developed diagnostic tests in order to identify and monitor the disease. In this simulated medical test, students will perform the ELISA to detect the presence of COVID-19 antibodies in simulated patient samples.

• Hands-on simulation of molecular testing for COVID-19
• Learn about the characteristics of coronaviruses
• Understand antibodies and immunoassays
• Perform an ELISA to detect the presence of COVID-19 antibodies in simulated patient samples

**Please note, this experiment is a simulation and intended solely for educational purposes. There is no virus or human blood products included with this experiment. The experiment will not detect SARS-CoV-2 antibodies in human blood samples.**


Group Size:

For 10 lab groups

Time Required:

Complete in 60 minutes

Kit Includes:

10X ELISA wash buffer, ELISA dilution buffer, antigen (lyophilized), primary & secondary antibodies (lyophilized), ABTS substrate (lyophilized), ABTS reaction buffer, microtiter plates, microcentrifuge tubes, & transfer pipets

All You Need:

Distilled or deionized water, beakers, pipet pump or bulb, lab glassware, disposable lab gloves, & safety goggles. Recommended: automatic micropipettes (0-50 µl, 100-1000 µl) and tips


Some components require refrigerator storage

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